For molecular diagnostic testing

FTA cards

An FTA card is a chemically treated filter paper designed for the collection of biological samples for subsequent DNA and RNA analysis. Special chemicals help to protect the DNA and RNA in addition to deactivating most harmful bacteria and viruses. FTA cards use FTA technology.

Sampling and purification

FTA technology simplifies the collection of biological samples and the purification of nucleic acids at room temperature. Following a few simple purification steps, DNA and RNA remains bound to the matrix of FTA cards, which can then be used directly for downstream applications. FTA chemistry has been optimized to lyse cells and denature proteins upon application of the biological sample onto the card to protect nucleic acids from nucleases, oxidation and UV damage. After drying, the released nucleic acids remain immobilized in the fibres of the matrix and are preserved for transport and later processing.

Advantages FTA cards

Submitting samples on FTA paper is easy and fast and samples can be submitted to our laboratory from any country.
The main benefits of using FTA cards are:
• No import permit is needed
• Submitting samples via regular post
• Low transportation costs
• Transportation at room temperature
• No risk for spreading infectious agents due to the chemicals included

Use of FTA cards is not recommended for monitoring, certification programs or assessment of disease free-status. FTA cannot be used to culture bacteria or viruses.

Taking samples

To obtain a sufficient amount of material for testing, press the inside of a cut slice of the tissue firmly on the FTA card or press the swab firmly on the FTA card. The FTA cards can be used with various tissue samples, swabs or blood (one spot can capture 500 μl whole blood). When there is a sufficient amount of material on the FTA card, the card will turn from pink to white. Allow the samples to dry at room temperature and normal humidity. Always wear gloves when handling the FTA cards to avoid contamination. Please label each spot with a code used for the identification of the sample.

Store unused cards in a dry, cool place and avoid humidity.

Ordering FTA cards

Please contact us if you want to order FTA cards. 

Submitting FTA cards

FTA cards can be submitted by post. The only document you should include is a GD submission form on which you mark the required analysis.
You can find all submission forms here.

Before submitting the FTA card(s), please make sure you put the dried samples through a microwave treatment. FTA cards should be heated for twenty seconds at 900 watt to inactivate any possible viruses. Warning: FTA Elute Micro Cards are not suitable for microwave treatment! Only use Classic Cards or Indicating Classic Cards.

Please submit FTA cards to:

GD Animal Health
Attention: CMD
Arnsbergstraat 7
7418 EZ Deventer
The Netherlands

Available tests

The following (RT-) PCR tests are available for poultry when sending samples by FTA card:

GD Catalogue  no.  (RT-) PCR test  Recommended material * (Swab) smear from Lead time in working days  Unit 
Viruses         
11466



 
Aviadenovirus I/Siadenovirus PCR (FTA card) pooled 1-4 organ (e.g. liver) or cloaca Per pool 
If Aviadeno PCR positive, the following test will be carried out: 11474 Adenovirus type I genotypering (FTA card)  20
 
 
11470


 
Aviadenovirus I/III EDS PCR (FTA card) pooled 1-4
organ (e.g. oviduct) or cloaca
Per pool 
If Aviadeno PCR positive, the following test will be carried out: 11474 Adenovirus type I genotypering (FTA card)  20   
11514 Avian influenza PCR (FTA card) pooled 1-4 Organ (e.g.trachea, choane) or cloaca Per pool
on request

Avian leukosis subgroeps A,B,C,D,J PCR (FTA card) pooled 1-4 blood, eggs, cloaca



Per pool

11444

Reovirus PCR (FTA card) pooled 1-4

Organ (e.g.intestine, tendonshaft) or cloaca; qualitative results Per pool

on request   If positive, genotyping can be carried out
11513
 
Infectious bronchitis virus PCR (FTA card) pooled 1-4

organs (e.g. trachea, kidney) or cloaca
 
6   Per pool
  If RT-PCR positive, the following test is carried out: 10606 Infectious bronchitis virus genotyping  20  
on request

IB PCR Massachusetts, 793B (FTA) pooled 1-4 

organs (e.g. trachea, kidney) or cloaca, quantitative results
 
14
 
Per sample
11515

 
Gumboro PCR (FTA card) pooled 1-4 Organ (e.g. bursa) 6 Per pool
If positive, the following test will be carried out: 10630 Gumboro (IBD) genotyping  20   
11592  Infectious laryngotracheitis PCR (FTA) pooled 1-4 Trachea, choane Per pool
11593 Turkey rhinotracheïtis (TRT) PCR (FTA) pooled 1-4  Trachea, choane  6 Per pool
11446 
Marek's disease virus PCR (FTA card) pooled 1-4 Organ (e.g. spleen, liver)  Per pool
on request  If positive, genotyping can be carried out       
11422





 
Intestinal pathogens PCR (FTA card) pooled 1-4
package containing:
Chicken Astrovirus 2
Chicken Astrovirus 3
Rotavirus A
Rotavirus D
Reovirus 
Cloacal samples duodenum/ jejenum, kidney; quantitative results



 
7




 
Per pool
              




Bacteria         
on request



        
Pakket Brachyspira PCR (FTA) pooled 1-4
containing:
B. intermedia,
B. pilosicoli and
B. hyodysenteriae 
ceacal droppings, culture



 
7



 
Per pool




-> on request  If positive, genotyping can be carried out       
on request
Chlamydia PCR (FTA) pooled 1-4 Organ (e.g. spleen, lung) or cloaca  7 Per pool 
on request Clostridium perfringens toxinotyping  Cloacal samples, intestine, livers, culture, droppings Per pool
11585 
Coryza (Avibacterium paragallinarum) PCR (FTA) pool 1-4  choane, sinus or culture  Per pool
-> on request   If positive, genotyping can be carried out 
     
on request  Histomonas meleagridis PCR (FTA) pooled 1-4  Organ (e.g. liver) cloacal or ceacal droppings  Per pool
11485   Mycoplasma gallisepticum PCR (FTA card) pooled 1-4  Trachea, choana, culture  Per pool
-> on request  If positive, genotyping can be carried out       
11591  Mycoplasma meleagridis PCR (FTA) pooled 1-4  Trachea, choana, culture  3 Per pool
11484 
Mycoplasma synoviae PCR (FTA card) pooled 1-4  Trachea, choana, culture  3 Per pool
-> on request  If positive, genotyping can be carried out       
11535 

Mycoplasma synoviae MS-1 PCR (FTA card) pooled 1-4
(differentiating vaccine from field strain) 
Tracheal, choanal, culture
 
3

Per pool

-> on request  If positive, genotyping can be carried out
     
11483 

Mycoplasma synoviae MS-H PCR (FTA card) pooled 1-4
(differentiating vaccine from field strain) 
Trachea, choana, culture
 
3
 
Per pool

-> on request   If positive, genotyping can be carried out       

* Other materials on request